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Deep-lipidotyping by mass spectrometry: recent technical advances and applications
Wenpeng Zhang1, Ruijun Jian2, Jing Zhao2
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, P. R. China.
Journal of Lipid Research
|April 30, 2022
Summary
Advanced mass spectrometry methods enable deep lipid typing, offering new ways to study lipid metabolism and identify biomarkers. This allows for detailed structural characterization beyond basic lipid identification.
Area of Science:
- Lipidomics
- Analytical Chemistry
- Biochemistry
Background:
- In-depth structural characterization of lipids is crucial for lipidomics.
- Mass spectrometry (MS/MS) methods have rapidly advanced for resolving lipid isomers.
- Deep lipid typing offers new avenues for studying lipid metabolism and biomarker discovery.
Purpose of the Study:
- To review recent advancements in tandem mass spectrometry (MS/MS) methods for complex lipid identification.
- To discuss identification of lipids beyond species and molecular species levels.
- To explore the integration and applications of isomer-resolving MS/MS in lipidomics.
Main Methods:
- Review of recent tandem mass spectrometry (MS/MS) techniques.
- Focus on isomer-resolving MS/MS for detailed lipid structural characterization.
- Integration of methods with lipid analysis workflows.
Main Results:
- MS/MS methods now identify lipids at C=C location and sn-position levels.
- Characterization of acyl chain modifications is now possible.
- Deep lipid typing enables untangling of individual isomer metabolism and sensitive phenotyping.
Conclusions:
- Advancements in MS/MS facilitate deep lipid typing for comprehensive lipidomics.
- Isomer-resolved lipid analysis provides new insights into lipid metabolism.
- Relative fractional quantitation of isomers aids in sensitive phenotyping and biomarker discovery.
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