Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

3.0K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
3.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.6K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

1.8K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
1.8K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

1.9K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
1.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

362
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
362
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

577
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
577

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Polarization Second Harmonic Generation Interferometry for Imaging Antiparallel Domains and Stacking Angles of 2D Heterocrystals.

ACS nano·2025
Same author

Unveiling the Mechanism of Plasmon Photocatalysis via Multiquantum Vibrational Excitation.

ACS nano·2024
Same author

Identifying the active sites and intermediates on copper surfaces for electrochemical nitrate reduction to ammonia.

Chemical science·2024
Same author

Exciton-Sensitized Second-Harmonic Generation in 2D Heterostructures.

ACS nano·2023
Same author

Real-Time Monitoring of a Single Molecule in Sub-nanometer Space by Dynamic Surface-Enhanced Raman Spectroscopy.

The journal of physical chemistry letters·2023
Same author

Role of Local Conductivities in the Plasmon Reflections at the Edges and Stacking Domain Boundaries of Trilayer Graphene.

The journal of physical chemistry letters·2023

Related Experiment Video

Updated: Sep 25, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.6K

Molecular vibrational imaging at nanoscale.

Boogeon Choi1, Gyouil Jeong1, Hyun-Hang Shin1

  • 1Department of Chemistry, Seoul National University, Seoul 08826, South Korea.

The Journal of Chemical Physics
|April 30, 2022
PubMed
Summary
This summary is machine-generated.

Visualizing chemical spatial distribution using vibrational spectra is crucial. This perspective discusses challenges and breakthroughs in nano-imaging techniques like scattering-type scanning near-field optical microscopy and tip-enhanced Raman scattering.

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.0K

Related Experiment Videos

Last Updated: Sep 25, 2025

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.6K
Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

2.0K

Area of Science:

  • Chemical analysis
  • Spectroscopy
  • Nanotechnology

Background:

  • Increasing demand for visualizing chemical spatial distribution using vibrational spectra.
  • Development of Raman and infrared spectro-microscopies with nanometric spatial resolution over the last two decades.

Purpose of the Study:

  • Highlight key challenges and recent breakthroughs in vibrational nano-imaging techniques.
  • Address the gap between general needs and current capabilities of these techniques.

Main Methods:

  • Review of scattering-type scanning near-field optical microscopy (s-SNOM).
  • Review of tip-enhanced Raman scattering (TERS).

Main Results:

  • Identified key challenges in achieving desired spatial resolution and sensitivity.
  • Highlighted recent advancements and breakthroughs in s-SNOM and TERS.

Conclusions:

  • Despite progress, a significant gap remains between the capabilities of vibrational nano-imaging techniques and the demands of chemical species visualization.
  • Further research and development are needed to overcome existing challenges and enhance the performance of s-SNOM and TERS.