Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Analysis of Multiple Serum Tumor Biomarkers by an Interpretable Stacked Ensemble Model.

Analytical chemistry·2026
Same author

Revealing Quantum Tunneling in a Nanocavity with Monolayer Graphene as a Stair.

The journal of physical chemistry letters·2026
Same author

Suppression of PCBM dimer formation in inverted perovskite solar cells.

Nature materials·2025
Same author

Correction to "Clean and Open Yo-Yo-Shaped Nanoparticle with Large and Sensitive Hot Space Enhanced Raman Spectroscopy in Ultradilute Colloids".

Analytical chemistry·2025
Same author

Attention Scale Fusion Network for Qualitative and Quantitative Analysis of Serum Tumor Biomarkers Via Label-Free Surface-Enhanced Raman Spectroscopy.

Analytical chemistry·2025
Same author

Clean and Open Yo-Yo-Shaped Nanoparticle with Large and Sensitive Hot Space Enhanced Raman Spectroscopy in Ultradilute Colloids.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.4K

Real-Time Monitoring of a Single Molecule in Sub-nanometer Space by Dynamic Surface-Enhanced Raman Spectroscopy.

Wuwen Yan1,2, Siyu Chen1,2, Pan Li1,3

  • 1Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, People's Republic of China.

The Journal of Physical Chemistry Letters
|September 22, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel platform using gold nanorods (GNRs) and surface-enhanced Raman spectroscopy (SERS) to monitor single molecules long-term. This breakthrough enables dynamic observation of molecular behavior without labels.

More Related Videos

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.4K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

2.7K

Related Experiment Videos

Last Updated: Jun 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.4K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.4K
Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy
10:59

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Published on: May 12, 2023

2.7K

Area of Science:

  • Biochemistry and Biophysics
  • Materials Science
  • Spectroscopy

Background:

  • Monitoring single molecules without labels is crucial for understanding biological and chemical processes.
  • Long-term observation of label-free molecules presents significant technical challenges.
  • Existing methods often struggle with sensitivity and duration for single-molecule analysis.

Purpose of the Study:

  • To develop a robust platform for long-term, label-free single-molecule monitoring.
  • To utilize the photothermal effect of gold nanorods (GNRs) for enhanced detection.
  • To capture dynamic molecular behavior at the single-molecule level.

Main Methods:

  • Fabrication of a platform based on gold nanorods (GNRs) utilizing their photothermal effect.
  • Employing dynamic surface-enhanced Raman spectroscopy (D-SERS) for molecular detection.
  • Utilizing laser re-irradiation to create optimal nanogaps (1.0 nm) between GNRs.
  • Performing bianalyte experiments at ultra-low concentrations (10-14 M).
  • Integrating density functional theory (DFT) calculations for molecular analysis.

Main Results:

  • Successful long-term monitoring (up to 4 min) of single crystal violet (CV) molecules.
  • Demonstration of single-molecule detection sensitivity at 10-14 M CV concentrations.
  • Observation of molecular blinking, indicating dynamic behavior.
  • Evidence of CV molecules confined to sub-nanometer spaces.
  • Dynamic capture of CV molecule orientation (50-90°) using D-SERS.

Conclusions:

  • The developed GNR-SERS platform enables unprecedented long-term, label-free single-molecule monitoring.
  • The study provides insights into molecular confinement and dynamic orientation.
  • This approach offers a novel strategy for exploring temporal and spatial dynamics in chemical reactions.