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MS imaging and absorption methods visualizing sun filter skin spatial distribution and penetration.

C Jacques1, F Crépel1, D El Assad2

  • 1Pierre Fabre Dermo-Cosmétique, Pierre Fabre R&D Center, Applied Research Department, Avenue Hubert Curien, Cedex 01, 31025 Toulouse, France.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|May 1, 2022
PubMed
Summary
This summary is machine-generated.

New sunscreens, including broad-spectrum TriAsorB, were studied for skin penetration. Both standard methods and advanced mass spectrometry imaging confirmed sun filters remain on the skin surface, not penetrating deeply.

Keywords:
Cutaneous distributionDamaged skinImage analysisMass spectrometry imagingSun filters

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Area of Science:

  • Dermatology
  • Cosmetic Science
  • Analytical Chemistry

Background:

  • Sunscreens require broad-spectrum protection against UV, visible, and infrared radiation.
  • Understanding sunscreen penetration is crucial for efficacy and safety.
  • Novel imaging techniques can offer deeper insights into topical product distribution.

Purpose of the Study:

  • To evaluate the skin penetration and distribution of a new broad-spectrum sunscreen filter, TriAsorB, combined with other sunscreens.
  • To compare the efficacy of traditional penetration protocols with advanced mass spectrometry imaging techniques.
  • To assess sunscreen distribution on both intact and damaged human skin.

Main Methods:

  • Formulation of TriAsorB with three other sunscreens.
  • Standard skin penetration protocol involving receptor fluid analysis.
  • Advanced mass spectrometry imaging: Atmospheric Pressure Matrix Assisted Laser Desorption Ionization (AP-MALDI) and Time of Flight - Secondary Ion Mass Spectrometry (ToF-SIMS).
  • Analysis of molecular distribution in cross-section human skin samples.

Main Results:

  • Standard penetration tests showed minimal absorption, with most sunscreen remaining on the skin surface.
  • Damaged skin did not exhibit increased sunscreen absorption.
  • Mass spectrometry imaging confirmed sun filters are primarily located on or within the stratum corneum.
  • Homogenous coverage of sun filters was observed across skin topography, correlating with natural skin biomarkers.
  • No sun filter compounds were detected in the receptor fluid.

Conclusions:

  • Conventional and novel imaging techniques demonstrate that sunscreens, including TriAsorB, predominantly reside on the skin's surface after topical application.
  • Mass spectrometry imaging provides a valuable, complementary tool for visualizing compound distribution and penetration in skin studies.
  • The findings suggest current sunscreen formulations may not significantly penetrate the deeper layers of the epidermis or dermis.