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Updated: Sep 25, 2025

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
Published on: October 19, 2019
Bead based facile assay for sensitive quantification of native state green fluorescent protein
Jung Min Kim1, Baik Lin Seong2, Dong-Kwon Lim1
1KU-KIST Graduate School of Converging Science and Technology, Korea University 145 Korea-ro Seongbuk-gu Seoul Republic of Korea dklim@korea.ac.kr.
Abstract:
A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.

