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Wrinkled material surfaces influence endothelial cell (EC) behavior and monocyte adhesion. Optimized topography (W10) enhances ECs and promotes monocyte interactions, aiding vascular repair research.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Vascular Biology

Background:

  • Endothelial cells (ECs) form a barrier crucial for vascular health.
  • EC dysfunction and monocyte adhesion contribute to inflammation and atherosclerosis.
  • Material surface topography can modulate cell behavior.

Purpose of the Study:

  • To investigate the impact of material wrinkled topographies on endothelial cell responses.
  • To examine the effect of these topographies on monocyte adhesion to endothelial cells.
  • To identify optimal surface dimensions for specific cellular responses.

Main Methods:

  • Fabrication of wrinkled surfaces with varying dimensions.
  • Culture of human umbilical vein endothelial cells (HUVECs) on these surfaces.
  • Assessment of HUVEC morphology, proliferation (EdU assay), and inflammatory cytokine expression.
  • Quantification of monocyte adhesion to HUVECs via ICAM and p-AKT expression.

Main Results:

  • HUVEC responses were non-linearly dependent on surface topography dimensions.
  • The W10 surface (3.5 μm amplitude, 10 μm wavelength) promoted HUVEC elongation and orientation.
  • HUVEC proliferation was highest on the W10 surface.
  • Increased inflammatory cytokine expression and monocyte adhesion were observed on the W10 surface, linked to p-AKT and ICAM levels.

Conclusions:

  • Material wrinkled topographies can effectively modulate endothelial cell behavior and monocyte interactions.
  • The W10 surface represents a promising topography for enhancing endothelial cell function and inflammatory responses.
  • This study provides an in vitro model for studying vascular diseases and testing therapies.