Related Experiment Video
Updated: Sep 25, 2025
07:02
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.5K
Silencing circOMA1 Inhibits Osteosarcoma Progression by Sponging miR-1294 to Regulate c-Myc Expression
Yubo Shi1, Yunyun Tian1, Xiangran Sun1
1Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
Frontiers in Oncology
|May 2, 2022
Summary
Circular RNA circOMA1 is upregulated in osteosarcoma, promoting tumor growth by sponging miR-1294 and increasing c-Myc. circOMA1 may be a therapeutic target for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are implicated in cancer development.
- The role of circOMA1 in osteosarcoma tumorigenesis remains uncharacterized.
Purpose of the Study:
- To investigate the expression and function of circOMA1 in osteosarcoma.
- To elucidate the molecular mechanism underlying circOMA1's role in osteosarcoma progression.
Main Methods:
- Bioinformatic analysis identified circOMA1.
- Quantitative reverse transcription PCR (qRT-PCR) assessed gene expression.
- In vitro and in vivo functional assays evaluated circOMA1's role.
- Luciferase reporter, RNA immunoprecipitation (RIP), and FISH assays confirmed molecular interactions.
Main Results:
- circOMA1 was significantly upregulated in osteosarcoma tissues.
- circOMA1 knockdown inhibited osteosarcoma cell proliferation and tumor growth.
- circOMA1 acts as a molecular sponge for miR-1294, enhancing c-Myc expression.
Conclusions:
- circOMA1 functions as an oncogene in osteosarcoma.
- The circOMA1/miR-1294/c-Myc pathway drives osteosarcoma progression.
- circOMA1 presents a potential therapeutic target for osteosarcoma treatment.
Related Concept Videos
01:22MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:06Induced Pluripotent Stem Cells
4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.4K
02:34Inhibition of Cdk Activity
5.0K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.0K
02:15Experimental RNAi
6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K