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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Sep 25, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking.

Tal Naor¹, Yevgeni Nogin^1,2, Elias Nehme^1,3

¹Department of Biomedical Engineering and Lokey Interdisciplinary Center for Life Sciences & Engineering, Technion-IIT, Haifa, 3200003, Israel.

|May 2, 2022

Summary

This summary is machine-generated.

Chromatin movement during the cell cycle is not constant. Researchers used advanced microscopy to show that telomere diffusion decreases in G0/G1 phases, revealing cell-cycle-dependent motion constraints.

Keywords:

Biological sciences Biophysics Chromosome organization Optical imaging

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Last Updated: Sep 25, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

Cell biology
Biophysics
Genomics

Background:

Understanding cell cycle progression and regulation is crucial for biophysics, aging, and disease research.
Local chromatin movements were traditionally thought to be consistent during interphase, but genome organization studies challenge this.
Telomere dynamics offer insights into genome organization and nuclear processes.

Purpose of the Study:

To characterize telomere diffusion during the interphase of mouse embryonic fibroblast (MEF 3T3) cells.
To investigate cell-cycle-dependent changes in chromatin dynamics and motion constraints.
To compare telomere diffusion in normal MEF 3T3 cells versus those lacking Lamin A (LmnaKO).

Main Methods:

Employed high spatiotemporal resolution 4D localization microscopy using point-spread-function (PSF) engineering.
Utilized deep learning-based image analysis for live imaging of MEF 3T3 and LmnaKO cell lines.
Quantified telomere diffusion and analyzed motion constraints across different cell cycle stages.

Main Results:

Detected varying levels of constraint on chromatin movement during the cell cycle.
Observed a prominent decrease in chromatin constraint during the G0/G1 phases.
Demonstrated that 4D telomere diffusion measurements can reveal cell-cycle-dependent motion constraints.

Conclusions:

Chromatin motion is not uniformly constrained throughout the cell cycle.
The G0/G1 phase exhibits significantly reduced constraints on chromatin movement.
4D telomere diffusion analysis provides a novel method for studying dynamic chromatin organization and its regulation.