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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Enterobacteria Phage SV76 Host Range and Genomic Characterization.

Caitlin M Carmody1, Emma L Farquharson1, Sam R Nugen1

  • 1Department of Food Science, Cornell University, Ithaca, NY 14853, USA.

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Summary

We sequenced and analyzed the genome of bacteriophage SV76, a T4-like phage. This phage shows a specific host range and utilizes FadL for infection, offering potential for biotechnological applications.

Keywords:
BacteriophageECORHost RangeSV76T2Whole Genome Sequencing

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Area of Science:

  • Microbiology
  • Genomics
  • Biotechnology

Background:

  • Bacteriophages are crucial for understanding biological processes and advancing biotechnology.
  • Phage SV76 is a T4-like bacteriophage within the Myoviridae family, genus Tequatrovirus.
  • Complete genomic data enhances knowledge of bacteriophage function and application.

Purpose of the Study:

  • To present the complete assembled and annotated genome sequence of bacteriophage SV76.
  • To determine the host range of SV76 against diverse Escherichia coli isolates.
  • To identify potential receptor binding proteins utilized by SV76.

Main Methods:

  • Whole genome sequencing, assembly, and annotation of SV76.
  • Double-agar spot assays to assess SV76 host range against 72 E. coli strains.
  • Analysis of E. coli K12 single gene knockouts to identify phage receptors.

Main Results:

  • The complete genome of SV76 was sequenced and annotated (Accession OM339528).
  • SV76 plaques 13.9% of tested E. coli strains and 100% of tested E. coli K12 knockouts.
  • SV76 infection requires the FadL protein, indicating its role as a receptor binding protein.

Conclusions:

  • SV76 shares genomic and host range similarities with the closely related phage T2.
  • This study provides novel genomic and host range data for SV76.
  • The findings lay the groundwork for future research into SV76 and other T4-like phages for biotechnological innovation.