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Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
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Leucomethylene blue probe detects a broad spectrum of reactive oxygen and nitrogen species
Christopher Cremer1, Jean Michél Merkes1,2, Christina L Bub1
1Institute of Organic Chemistry, RWTH Aachen University Landoltweg 1 Aachen Germany.
RSC Advances
|May 2, 2022
Abstract:
Reactive oxygen and nitrogen species (ROS, RNS) are ubiquitous in biology with a variety of physiological and pathological functions. Here we describe a broad spectrum ROS/RNS detecting fluorogenic probe with red fluorescence emission and up to 100-fold gain. Hence these modified probes are useful for in vivo non-invasive quantification of ROS/RNS.

