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Essential oil loaded pectin/chitosan nanoparticles preparation and optimization via Box-Behnken design against MCF-7
Olivia A Attallah1,2, Amro Shetta1, Fatma Elshishiny1
1Department of Chemistry, School of Sciences and Engineering (SSE), The American University in Cairo (AUC) AUC Avenue, P.O. Box 74 New Cairo 11835 Egypt wael_mamdouh@aucegypt.edu.
Abstract:
In the continuous search for effective cancer treatments, we here report a novel anticancer nanoparticulate system composed of jasmine oil (JO), an essential oil with proven anticancer activity and pectin/chitosan composite nanoparticles (Pec/CS NPs) as encapsulating materials to overcome JO's solubility and sensitivity problems using a green ionotropic gelation method. Pec/CS/JO NPs were formulated using Box-Behnken design (BBD) to estimate the interactions and effects of studied formulation variables on particle size, zeta potential and encapsulation efficiency to develop an optimized Pec/CS nanoformulation. The nano-encapsulation system preserved the consistency of total phenolic contents in JO and amended its thermal stability by 1.64 fold. The antioxidant potency of JO was enhanced after encapsulation by 96.28%. Consequently, the cytotoxic activity of bare Pec/CS NPs, pure JO and encapsulated JO in Pec/CS NPs against (MCF-7) breast cancer cells and (L-929) normal cells was evaluated using MTT assay. Encapsulated JO was more potent than pure JO with ≈13 fold improvement in anticancer activity, whereas the cell viability of normal cells wasn't affected but was rather enhanced when treated with Pec/CS NPs.
Insights
Jasmine oil (JO) was encapsulated in pectin/chitosan nanoparticles (Pec/CS NPs) to create a novel anticancer system. This enhanced JO
Area of Science:
- Nanotechnology
- Materials Science
- Pharmacology
Background:
- Jasmine oil (JO) exhibits anticancer properties but faces challenges with solubility and stability.
- Essential oils require effective delivery systems for therapeutic applications.
- Pectin/chitosan composite nanoparticles (Pec/CS NPs) offer a biocompatible platform for drug delivery.
Purpose of the Study:
- To develop a novel nanoparticulate system for jasmine oil (JO) using pectin/chitosan composite nanoparticles (Pec/CS NPs).
- To enhance the anticancer efficacy and stability of JO.
- To optimize the formulation of Pec/CS/JO NPs using Box-Behnken design (BBD).
Main Methods:
- Fabrication of Pec/CS/JO NPs via a green ionotropic gelation method.
- Optimization of nanoparticle formulation using Box-Behnken design (BBD) to assess particle size, zeta potential, and encapsulation efficiency.
- Evaluation of total phenolic content, thermal stability, antioxidant activity, and cytotoxic effects using MTT assay.
Main Results:
- The nano-encapsulation preserved JO's phenolic content and increased its thermal stability by 1.64-fold.
- Antioxidant potency of JO was significantly enhanced by 96.28% after encapsulation.
- Encapsulated JO demonstrated a ~13-fold improvement in anticancer activity against MCF-7 breast cancer cells compared to pure JO, with no adverse effects on normal L-929 cells.
Conclusions:
- The developed Pec/CS/JO NPs represent a promising strategy for enhancing the therapeutic potential of jasmine oil.
- This novel nanoformulation effectively delivers JO, improving its anticancer efficacy and stability.
- The study highlights the potential of pectin/chitosan nanoparticles as a drug delivery vehicle for natural anticancer agents.
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