Related Experiment Video
Updated: Sep 25, 2025
09:34
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
Published on: July 30, 2016
21.4K
Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
Vicky Bronnec1, Oleg A Alexeyev1
1Department of Medical Biosciences/Pathology, Umeå University, 90 187 Umeå, Sweden.
STAR Protocols
|May 2, 2022
Abstract:
In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly's gut using an easy to carry out method. For complete details on the use and execution of this protocol, please refer to Bronnec and Alexeyev (2021) and Bronnec et al. (2022).
