Longitudinal two-photon calcium imaging with ultra-large cranial window for head-fixed mice
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Updated: Sep 25, 2025
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
Published on: August 4, 2022
Ryoma Hattori1, Takaki Komiyama1
1Neurobiology Section, Center for Neural Circuits and Behavior, Department of Neurosciences, and Halıcıoğlu Data Science Institute, University of California, San Diego, La Jolla, CA 92093, USA.
Abstract:
Neural activity is heterogeneous across different cortical areas and can change during learning. Here, we describe a protocol for longitudinal in vivo two-photon calcium imaging with an ultra-large cranial window that exposes most of the dorsal cortex in head-fixed mice. The large cranial window allows optical access to any dorsal cortical areas in individual mice. This protocol enables longitudinal tracking of neural activity from various cortical areas at cellular resolution to understand the cortical computations during behavioral tasks. For complete details on the use and execution of this protocol, please refer to Hattori et al. (2019), and Hattori and Komiyama, 2022a.
