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Related Experiment Video

Updated: Sep 25, 2025

Polysome Purification from Soybean Symbiotic Nodules
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High-Resolution Translatome Analysis Reveals Cortical Cell Programs During Early Soybean Nodulation.

Jae Hyo Song1, Bruna Montes-Luz1, Michelle Zibetti Tadra-Sfeir1

  • 1Divisions of Plant Sciences and Biochemistry, Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, United States.

Frontiers in Plant Science
|May 2, 2022
PubMed
Summary

This study used translating ribosome affinity purification (TRAP) to identify early gene responses in the legume root cortex during nodulation. Novel nodulation genes and phytohormone signaling pathways were discovered, advancing our understanding of this crucial symbiotic process.

Keywords:
TRAP-seqcortical cellnodulationphytohormonesoybean

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Area of Science:

  • Plant Molecular Biology
  • Legume Symbiosis
  • Genomics

Background:

  • Nodule organogenesis in legumes involves complex gene networks, but previous studies faced challenges due to tissue dilution.
  • Understanding early root cortex responses to rhizobial infection is limited.

Purpose of the Study:

  • To identify genes specifically regulated during early nodule organogenesis in the legume root cortex.
  • To investigate the role of phytohormone signaling in early nodule development.

Main Methods:

  • Translating ribosome affinity purification (TRAP) with a cortex-specific promoter to isolate responding cells.
  • Inoculation of soybean roots with *Bradyrhizobium japonicum*.
  • Promoter-GUS analysis and RNA interference (RNAi) for gene validation and functional studies.

Main Results:

  • Identified novel nodulation gene candidates and soybean orthologs of known nodulation genes.
  • Confirmed differential cortex expression of several genes.
  • Discovered differentially regulated genes involved in auxin, cytokinin, and gibberellic acid signaling.

Conclusions:

  • TRAP effectively monitored early root cortex responses to rhizobial inoculation.
  • This study provides new insights into phytohormone signaling during early nodule development in legumes.