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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Differential expression profile of plasma exosomal microRNAs in chronic rhinosinusitis with nasal polyps
Abstract:
Exosomes mediate inflammation and immune responses. The aim of the study was to examine the expression profiles of plasma exosomal microRNAs (miRNAs) and analyze their target gene functions in participants with chronic rhinosinusitis with nasal polyps (CRSwNP). We measured plasma exosomal miRNAs in five patients with CRSwNP and five controls. Transcripts per million (TPM) was used to assess miRNAs expression and the Benjamini-Hochberg procedure was employed for multiple comparisons correction. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene ontology (GO) analyses revealed biological annotation and functional prediction of target genes. Compared with controls, we found that 159 exosomal miRNAs were differentially expressed by miRNA sequencing in CRSwNP. The top three upregulated miRNAs were novel_miR_677, novel_miR_1037, and novel_miR_79, while the top three downregulated miRNAs were novel_miR_192, novel_miR_1022, and novel_miR_4. The target functions in the GO and KEGG analyses included axon guidance, extracellular matrix (ECM)-receptor interaction, protein digestion and absorption, the calcium, the Hippo, the Notch, the ErbB, the cAMP signaling pathway, and focal adhesion. This study describes the dissection of plasma exosomal miRNA profiling in CRSwNP. Our findings may provide a certain basis for further mechanism research and exploration of diagnostic values.
Insights
This study reveals distinct plasma exosomal microRNA (miRNA) profiles in chronic rhinosinusitis with nasal polyps (CRSwNP), identifying specific miRNAs and their functions that may aid in understanding disease mechanisms and diagnostics.
Area of Science:
- Biochemistry
- Immunology
- Genetics
Background:
- Exosomes play a role in inflammation and immune responses.
- Chronic rhinosinusitis with nasal polyps (CRSwNP) is an inflammatory upper airway disease.
- MicroRNAs (miRNAs) are key regulators of gene expression and are implicated in various diseases.
Purpose of the Study:
- To investigate the expression profiles of plasma exosomal miRNAs in patients with CRSwNP.
- To analyze the functional roles of target genes associated with these exosomal miRNAs.
- To identify potential biomarkers for CRSwNP.
Main Methods:
- Plasma exosomal miRNAs were isolated from five CRSwNP patients and five healthy controls.
- miRNA sequencing was performed to quantify miRNA expression levels (Transcripts Per Million, TPM).
- Bioinformatic analyses, including Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO), were used for functional annotation.
Main Results:
- 159 exosomal miRNAs were found to be differentially expressed in CRSwNP patients compared to controls.
- Key upregulated miRNAs included novel_miR_677, novel_miR_1037, and novel_miR_79.
- Downregulated miRNAs included novel_miR_192, novel_miR_1022, and novel_miR_4.
- Enriched target gene functions involved pathways such as axon guidance, ECM-receptor interaction, and signaling pathways (Hippo, Notch, cAMP).
Conclusions:
- Plasma exosomal miRNA profiling provides a detailed molecular signature for CRSwNP.
- The identified differentially expressed miRNAs and their target pathways offer insights into CRSwNP pathogenesis.
- These findings suggest potential for exosomal miRNAs as diagnostic biomarkers for CRSwNP.
