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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: Sep 24, 2025

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

iPS Cell Differentiation into Brain Microvascular Endothelial Cells.

Angelica Medina¹, Hengli Tang²

¹Department of Biological Science, Florida State University, Tallahassee, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 4, 2022

Summary

This study details a method to create brain endothelial cells from stem cells using defined culture components. These cells mimic the blood-brain barrier, offering a scalable model for research and drug discovery.

Keywords:

Barrier integrity Blood–brain barrier Endothelial cells Stem cells TEER

More Related Videos

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Published on: November 19, 2020

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Published on: January 23, 2014

Related Experiment Videos

Last Updated: Sep 24, 2025

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Published on: November 19, 2020

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Published on: January 23, 2014

Area of Science:

Neuroscience
Cell Biology
Biotechnology

Background:

The blood-brain barrier (BBB) protects the central nervous system from toxins and plasma fluctuations.
It comprises endothelial cells with tight junctions, supported by pericytes and astrocytes.
BBB disruption is linked to central nervous system pathogenesis, making its study crucial.

Purpose of the Study:

To develop a reproducible and scalable method for generating brain microvascular endothelial cells (BMECs).
To create functional BMECs with barrier characteristics from stem cells.
To provide a reliable cell source for BBB research, drug discovery, and pathology studies.

Main Methods:

Utilizing defined serum components in stem cell culture to provide specific cellular cues.
Inducing differentiation of stem cells towards a brain endothelial cell lineage.
Establishing protocols for reproducible and scalable cell culture.

Main Results:

Successful differentiation of stem cells into BMECs with demonstrated barrier characteristics.
Achieved reproducible and scalable cultures of functional BMECs.
Validated the potential for long-term storage and cryopreservation of differentiated BMECs.

Conclusions:

The described method provides a robust protocol for generating functional BMECs from stem cells.
This approach facilitates the study of blood-brain barrier structure, function, and disruption.
The generated BMECs are suitable for various applications, including drug screening and disease modeling.