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Recent developments in miRNA based recombinant protein expression in CHO.

Masoume Bazaz1, Ahmad Adeli1, Mohammad Azizi1

  • 1Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

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MicroRNAs are a promising tool for engineering mammalian cell lines, like Chinese hamster ovary cells, to boost the production of recombinant therapeutic proteins. This review covers methods and applications of microRNA-based strategies for enhanced protein expression.

Keywords:
BiopharmaCHOCell line developmentFed batch cultureGene silencingProductivityRecombinant proteinmicroRNA

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Area of Science:

  • Biotechnology and Genetic Engineering
  • Molecular Biology
  • Biopharmaceutical Production

Background:

  • The biopharmaceutical industry is expanding due to increased demand for recombinant therapeutic proteins.
  • Mammalian cell lines, especially Chinese hamster ovary (CHO) cells, are crucial for producing these proteins.
  • Traditional methods focus on genetic manipulation of cellular processes to enhance host cell productivity.

Purpose of the Study:

  • To review microRNA (miRNA) based strategies for engineering mammalian host cells.
  • To explore methods for selecting target miRNAs for cell line engineering.
  • To discuss the application of miRNA gain- or loss-of-function strategies in enhancing protein expression.

Main Methods:

  • Review of literature on miRNA-based genetic engineering tools for biopharmaceutical production.
  • Analysis of methods for identifying and selecting target miRNAs.
  • Examination of miRNA gain-of-function and loss-of-function approaches in cell line engineering.

Main Results:

  • MicroRNAs can simultaneously target multiple mRNAs, making them effective for modulating complex cellular pathways.
  • Various studies demonstrate the potential of miRNAs to increase recombinant protein expression levels in host cells.
  • The review covers examples from laboratory and pilot studies showcasing miRNA applications.

Conclusions:

  • MicroRNAs represent a powerful tool for genetic engineering of mammalian cell lines to improve recombinant protein production.
  • The technology offers advantages in simultaneously regulating multiple targets for enhanced protein expression.
  • Discussion includes the benefits and drawbacks of using miRNAs in biopharmaceutical cell line development.