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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

661
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
661
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

554
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
554

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Related Experiment Video

Updated: Sep 24, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
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Surface-enhanced Raman scattering: An emerging tool for sensing cellular function.

Swati Tanwar1, Jeong Hee Kim1, Jeff W M Bulte2,3,4,5,6

  • 1Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|May 5, 2022
PubMed
Summary
This summary is machine-generated.

Surface-enhanced Raman scattering (SERS) offers sensitive, molecule-specific intracellular imaging and multiplexed monitoring. This powerful spectroscopic tool enables real-time tracking of cellular processes, surpassing traditional methods for biological applications.

Keywords:
Cellular imaging and sensingPlasmonic nanoparticlesSERS

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Area of Science:

  • Nanotechnology approaches to biology
  • Diagnostic tools (in vitro, in vivo, biosensing)

Background:

  • Continuous long-term intracellular imaging and multiplexed monitoring of biomolecular changes are significant challenges.
  • Conventional imaging methods often lack the sensitivity and specificity required for dynamic cellular process analysis.

Purpose of the Study:

  • To critically discuss recent developments in surface-enhanced Raman scattering (SERS) for biological applications.
  • To highlight SERS's potential for intracellular imaging, multiplexed monitoring, and surpassing limitations of conventional methods.

Main Methods:

  • Utilizing surface-enhanced Raman scattering (SERS) as a spectroscopic tool.
  • Employing novel Raman reporters with narrow spectral peaks for enhanced multiplex sensing.
  • Focusing on SERS applications for biological sensing, molecular imaging, and dynamic intracellular process monitoring.

Main Results:

  • SERS provides molecule-specific information with high sensitivity, suitable for small sample volumes and real-time monitoring.
  • SERS-based probes enable targeted multiplex sensing, outperforming fluorescent probes in detection sensitivity.
  • SERS is a non-destructive technique applicable to biological sensing, molecular imaging, and dynamic monitoring.

Conclusions:

  • Surface-enhanced Raman scattering (SERS) is a powerful, non-destructive spectroscopic technique for biological applications.
  • SERS offers significant advantages for intracellular imaging and multiplexed monitoring of cellular processes.
  • SERS shows great potential for in vivo human applications in diagnostics and imaging.