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Updated: Sep 24, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Complete Genome Sequence of Stenotrophomonas maltophilia Phage Philippe

Iris G Vallavanatt1, Mariah Bartz1, James Clark1,2

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|May 5, 2022
PubMed

Insights

Stenotrophomonas maltophilia podophage Philippe, a novel virus, has a large genome related to N4-like phages. Its low sequence identity suggests it represents a new, unclassified member of the Rothmandenesvirinae subfamily.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Stenotrophomonas maltophilia is an opportunistic pathogen known for multidrug resistance.
  • Bacteriophages, viruses that infect bacteria, are increasingly studied for their potential applications.

Purpose of the Study:

  • To characterize the genome of Stenotrophomonas maltophilia podophage Philippe.
  • To determine the phylogenetic relationship of phage Philippe to other known bacteriophages.

Main Methods:

  • Whole-genome sequencing of Stenotrophomonas maltophilia podophage Philippe.
  • Bioinformatic analysis for sequence identity comparison and phylogenetic placement.

Main Results:

  • The genome size of phage Philippe is 74,717 bp.
  • Phage Philippe shares broad genomic relation to the N4-like phage group, including Stenotrophomonas phage Pokken.
  • Low sequence identity to related phages indicates Philippe is a distinct lineage.

Conclusions:

  • Phage Philippe is a novel S. maltophilia virus with a large genome.
  • Phage Philippe is likely an unclassified member of the N4-like phage subfamily Rothmandenesvirinae.

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