Impact of magnesium ions on lysozyme-triggered disintegration and solubilization of waste activated sludge

Lysozyme can efficiently accelerate solubilization and hydrolysis of waste activated sludge (WAS) for anerobic digestion. However, the effect of lysozyme was easily to be inhibited by metal ions in WAS. The impact of magnesium ions (Mg²⁺) on lysozyme catalyze WAS disintegration was investigated in this study. The effect of lysozyme on WAS hydrolysis could be hindered by Mg²⁺. Relatively high concentrations (>50 mg/L) of Mg²⁺ in sludge significantly reduced the release of soluble polysaccharides and proteins from WAS, while sulfate ions or chloride ions caused no such effect. Proteins were difficult to be extracted from extracellular polymeric substances (EPS) of WAS in the presence of Mg²⁺ (>10 mg/L) due to the divalent cation bridging (DCB) behavior, while the extraction of polysaccharides was not significantly affected. The polysaccharides and proteins in the inner EPS layer were transferred to the outer layer during the lysozyme treatment, and total quantities of both components maintained constantly. At least 23.1% lysozymes were trapped in the liquid phase of 100 mg Mg²⁺/L in the first hour. Mg²⁺ could significantly affect the transfer of lysozyme from liquid phase to the inner layer of sludge. Mg²⁺ neutralized the negative surface charge of the sludge particles, which hindered the absorption of positively charged lysozyme molecules by sludge flocs from the liquid phase. The proteins of TB-EPS had higher ratios of α-helixes and tighter structures than those in LB-EPS, which could impede the lysozyme transfer to the cell wall.