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Updated: Aug 4, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Biodiesel production in a magnetically fluidized bed reactor using whole-cell biocatalysts immobilized within
Jing Liu1, Guanyi Chen2, Beibei Yan3
1School of Agricultural Engineering and Food Science, Shandong Research Center of Engineering & Technology for Clean Energy, Shandong University of Technology, Zibo 255000, China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
Abstract:
Magnetic whole-cell biocatalysts (MWCBs) constructed by immobilizing Bacillus subtiliscells within ferroferric oxide-polyvinyl alcohol composite beads were developed and employed to transesterify waste frying oil to biodiesel in a magnetically fluidized bed reactor (MFBR). Effective variables including biocatalysts concentration, reactant flow rate, magnetic field intensity and temperature were evaluated to enhance the transesterification. By coupling MFBR with MWCBs, continuous biodiesel production was achieved. Response surface methodology and Box-Behnken design were employed to predict the optimal conditions and the maximum biodiesel yield reached 89.0 ± 0.6% after 48 h under the optimized conditions. Furthermore, MWCBs displayed satisfactory stability and reusability in MFBR and still maintained a biodiesel yield of more than 82.5% after 10 cycles. Lastly, the fuel properties of the obtained biodiesel met the ASTM and EN standards. The present study revealed that the route of producing biodiesel over MWCBs in the MFBR system showed great potential for industrialization.
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