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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection.

Jeunice Ida¹, Akinori Kuzuya², Yee Siew Choong¹

¹Institute for Research in Molecular Medicine, Universiti Sains Malaysia 11800 Penang Malaysia theamsoon@usm.my +60-4-653-4803 +60-4-653-4852.

|May 6, 2022

Summary

This study introduces a novel G-quadruplex (G4) DNAzyme method for distinguishing dengue virus (DENV) serotypes. The colorimetric assay utilizes split G4 configurations for sensitive and specific DENV detection.

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Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

Nucleic acids form unique structures like G-quadruplex (G4) DNA, enabling diagnostic and therapeutic applications.
Dengue virus (DENV) poses a significant global health challenge, necessitating accurate and rapid diagnostic tools.

Purpose of the Study:

To develop a G-quadruplex (G4) based DNAzyme assay for distinguishing dengue virus (DENV) serotypes.
To create a colorimetric sensing platform for DENV detection utilizing a split G4-hemin DNAzyme configuration.

Main Methods:

Utilized a split G4-hemin DNAzyme configuration where two oligonucleotides hybridize to form a G4 structure upon target DNA binding.
Employed a DNAzyme that catalyzes the oxidation of ABTS by H2O2, resulting in a color change for colorimetric detection.
Incorporated betaine and dimethyl sulfoxide to enhance target-probe hybridization and G4 formation.

Main Results:

Demonstrated the ability of the split G4-hemin DNAzyme system to differentiate between DENV serotypes (DENV-1, -2, -3, and -4).
Achieved colorimetric detection of DENV through the catalytic activity of the formed G4-hemin DNAzyme.
Successfully examined a multi-probe cocktail assay for simultaneous DENV detection.

Conclusions:

The split G-quadruplex configuration offers a promising approach for developing sensitive and serotype-specific DNA-based detection systems for viruses like DENV.
This method highlights the potential of G4 DNAzyme technology for future diagnostic applications, including rapid serotyping.