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Related Experiment Video

Updated: Sep 24, 2025

Fabrication of Fine Electrodes on the Tip of Hypodermic Needle Using Photoresist Spray Coating and Flexible Photomask for Biomedical Applications

Fabrication of Fine Electrodes on the Tip of Hypodermic Needle Using Photoresist Spray Coating and Flexible Photomask for Biomedical Applications

Published on: November 28, 2017

Microfabricated needle for hydrogen peroxide detection.

Shilun Feng^1,2, Sandhya Clement^2,3, Yonggang Zhu^4,5

¹School of Engineering, Macquarie University Sydney NSW 2109 Australia shilun.feng@gmail.com.

|May 6, 2022

Summary

This summary is machine-generated.

A novel microfluidic probe enables continuous, on-site monitoring of hydrogen peroxide (H2O2) levels. This system precisely quantifies H2O2 concentrations by analyzing fluorescent droplet reactions for analytical and biological applications.

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Area of Science:

Analytical Chemistry
Microfluidics
Chemical Sensing

Background:

Accurate monitoring of hydrogen peroxide (H2O2) is crucial in various chemical and biological processes.
Existing methods may lack the sensitivity or continuous monitoring capabilities required for dynamic reactions.

Purpose of the Study:

To develop and characterize a microfluidic system for real-time sampling and detection of H2O2.
To enable on-site, continuous quantitative analysis of H2O2 concentrations.

Main Methods:

Design and fabrication of a microfluidic needle-like probe.
Integration with a commercial, single-step fluorescent H2O2 assay.
Utilizing a mineral-oil carrier fluid for reagent droplet delivery.
Sample introduction via hydrophilic capillaries under negative pressure.
Droplet merging, reaction, and fluorescence-based detection.

Main Results:

Demonstrated successful on-site continuous sampling and controlled reaction of H2O2.
Characterized assay fluorescence response to variations in sample volume.
Established a calibration method to quantify H2O2 concentration from droplet size and intensity.
Developed a microfluidic data-logger system for signal storage and online detection.

Conclusions:

The developed microfluidic probe system provides a robust tool for real-time H2O2 monitoring.
This technology is applicable for analyzing dynamic chemical reactions in analytical chemistry and biological fields.
Offers a valuable solution for continuous, quantitative detection in various applications.