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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
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Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
Yang Wang1, Wei Wang2, Zhun Gu1
1Department of Medical Technology, Suzhou Chien-shiung Institute of Technology Taicang 215411 Jiangsu Province P. R. China 0070@csit.edu.cn.
RSC Advances
|May 6, 2022
Summary
New iron (Fe)-based coordination polymer nanohydrogels (FeCPNGs) were developed as nanozymes. These FeCPNGs exhibit dual peroxidase and superoxide dismutase (SOD) enzyme-like activities for biomedical applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Catalysis
Background:
- Iron (Fe)-based nanozymes are valuable in biomedicine due to their catalytic properties.
- Developing novel nanozymes with enhanced functionalities is crucial for advanced biomedical applications.
Purpose of the Study:
- To synthesize and characterize novel Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) as nanozymes.
- To evaluate the peroxidase and superoxide dismutase (SOD) mimetic activities of the synthesized FeCPNGs.
Main Methods:
- FeCPNGs were prepared via chelation of ferrous iron with esterified P(VCL-co-NMAM) nanohydrogels.
- Characterization involved dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).
- Enzyme-like activities were assessed using TMB/H2O2 oxidation for peroxidase activity and a commercial kit for SOD-like activity.
Main Results:
- Optimized nanohydrogels (4% monomer concentration, 1:1 VCL:NMAM ratio) showed uniform particle size, good dispersion, and temperature responsiveness.
- Characterization confirmed the successful synthesis of esterified nanohydrogels and FeCPNGs.
- FeCPNGs demonstrated significant peroxidase and SOD mimetic activities, mimicking natural enzymes.
Conclusions:
- Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) represent a promising new class of nanozymes.
- These FeCPNGs possess dual peroxidase and SOD-like activities, indicating potential for biomedical applications.
- The study highlights the successful development of functional nanozymes through coordination polymer chemistry.
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