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Modified enzyme immunoassays for tobramycin using reduced sample and reagent volumes
American Journal of Hospital Pharmacy
|March 1, 1987
Summary
Two modified enzyme immunoassay (EMIT) methods using reduced sample volumes (25- and 30-microL) accurately determine serum tobramycin concentrations. These cost-effective EMIT assays maintain accuracy comparable to the standard 50-microL method.
Area of Science:
- Clinical Chemistry
- Pharmacokinetics
- Analytical Toxicology
Background:
- Accurate monitoring of serum tobramycin concentrations is crucial for therapeutic efficacy and minimizing toxicity.
- Standard enzyme immunoassay (EMIT) methods require significant sample and reagent volumes, impacting cost and throughput.
Purpose of the Study:
- To evaluate the accuracy and reliability of two modified EMIT methods with reduced sample and reagent volumes (25-microL and 30-microL) for serum tobramycin determination.
- To compare the performance of these modified methods against the standard 50-microL EMIT assay.
Main Methods:
- Assayed known concentrations of tobramycin sulfate (1-12 mg/L) using standard (50-microL) and modified (25-microL, 30-microL) EMIT methods.
- Analyzed fourteen patient blood samples with unknown tobramycin concentrations using all three methods.
- Calculated mean percentage recovery and correlation coefficients (r) to assess accuracy and agreement.
Main Results:
- Mean percentage recovery for the 50-microL (104.4%), 30-microL (106.6%), and 25-microL (100.4%) methods were not significantly different.
- Excellent correlation was observed between the standard method and the modified methods (r = 0.993 for 25-microL; r = 0.988 for 30-microL).
Conclusions:
- Modified EMIT assays using reduced sample and reagent volumes (25-microL and 30-microL) provide accurate serum tobramycin concentration measurements.
- These optimized methods offer substantial cost savings and increased assay throughput without compromising analytical accuracy.