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Proteomics datasets of developing rat brain: Synaptic proteome and SUMO2/3-ylome
Félicie Kieffer1, Marie Pronot1, Anne-Sophie Gay1
1Université Côte d'Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, France.
Data in Brief
|May 6, 2022
Summary
This study characterizes the synaptic proteome and identifies Small Ubiquitin-like MOdifier (SUMO)-modified proteins in the developing rat brain. These findings offer insights into synaptic development and potential brain pathologies.
Area of Science:
- Neuroscience
- Proteomics
- Molecular Biology
Background:
- Synaptic development involves structural and functional changes.
- Post-translational modifications, such as SUMOylation, are key molecular processes.
- SUMOylation plays a crucial role in regulating synaptic function.
Purpose of the Study:
- To characterize the synaptic proteome in the developing rat brain at postnatal day 14 (PND14).
- To identify endogenous SUMO2/3-modified proteins within the synaptic compartment.
- To provide a comprehensive list of SUMOylated proteins in developing synapses.
Main Methods:
- Synaptosomal fraction isolation using differential centrifugation and sucrose-Percoll gradient.
- Proteomic analysis of the synaptic proteome via nanoLC-MS/MS.
- Enrichment of SUMO2/3-ylated proteins using denaturing immunoprecipitation.
Main Results:
- Detailed characterization of the synaptic proteome at PND14.
- Identification of numerous SUMO2/3-modified proteins in the developing rat brain synapses.
- Establishment of a comprehensive catalog of the synaptic SUMOylome.
Conclusions:
- The identified synaptic proteome and SUMOylated proteins provide a foundation for understanding synaptic development.
- These data can guide future research on the functional roles of SUMOylation in synapse structure and function.
- The findings may contribute to mechanistic studies of brain pathologies associated with altered SUMOylation levels.
