Enhanced photoelectrochemical biosensing performance for Au nanoparticle-polyaniline-TiO₂ heterojunction composites

A new photoelectrochemical (PEC) sensing platform comprising TiO₂ nanotube arrays (TiONTAs), polyaniline (PANI), and gold nanoparticles (AuNPs) was successfully fabricated. After loading the enzyme, this Au-PANI-TiONTA electrode showed excellent response to glucose at a linear range of 2-36 mM with a 0.02 mM detection limit. Good PEC performance was obtained due to the following advantages of the material: high visible-light harvesting ability for excellent light trapping capacity of PANI and AuNPs, good separation of the photo-induced charges related to the specific Au-PANI-TiONTA heterostructure, efficient electrode surface reaction kinetics derived from the large specific surface area of TiONTAs and improved electrode catalytic activity. This work proposed a new and general PEC enzymatic format and can be extended to prepare different PEC biosensors for biomolecules such as DNA, proteins and substrates of oxidases.