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Updated: Sep 24, 2025

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

Published on: September 8, 2023

Smartphone-based kanamycin sensing with ratiometric FRET.

Saurabh Umrao¹, Anusha S¹, Vasundhara Jain¹

¹Department of Chemical Engineering, Indian Institute of Science Bangalore 560012 India rahulroy@iisc.ac.in +91-80-2360-8121 +91-80-2293-3115 +91-80-2293-3118.

|May 6, 2022

Summary

This summary is machine-generated.

This study introduces a smartphone-based biosensor for sensitive kanamycin detection using Förster resonance energy transfer (FRET) and DNA aptamers. The developed kanamycin binding aptamer (KBA) sensor offers rapid, specific, and quantitative on-site analysis.

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Last Updated: Sep 24, 2025

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Molecular Biology

Background:

Smartphone-based fluorescence detection offers potential for on-site biosensing.
Quantitative field analysis is hindered by excitation and calibration challenges.
Förster resonance energy transfer (FRET) offers a promising approach for sensitive detection.

Purpose of the Study:

To develop a rapid and sensitive method for kanamycin detection using smartphone-based fluorescence.
To utilize ratiometric analysis with FRET between dye pairs on DNA aptamers for quantitative detection.
To enable on-site, specific, and repeatable kanamycin detection in complex matrices like milk.

Main Methods:

Developed a FRET-based kanamycin binding aptamer (KBA) sensor utilizing ligand binding-induced aptamer structural changes.
Employed ratiometric analysis for quantitative fluorescence detection.
Integrated the KBA sensor with a custom-built smartphone setup for field analysis.

Main Results:

The KBA sensor demonstrated two linear ranges for kanamycin detection: 0.05–5 nM (LOD 0.18 nM) and 50–900 nM.
The aptamer exhibited high specificity, even in the presence of milk background.
The smartphone-based FRET aptamer scheme achieved a linear range of 50–500 nM with an LOD of 28 nM.

Conclusions:

Ratiometric FRET analysis on DNA aptamers enables rapid, sensitive, and quantitative kanamycin detection.
The smartphone-based KBA sensor is suitable for on-site, specific, and repeatable analysis.
This approach overcomes limitations of traditional field fluorescence detection methods.