Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:34

DNA Isolation

194.5K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
194.5K
Diamagnetic Shielding of Nuclei: Local Diamagnetic Current01:14

Diamagnetic Shielding of Nuclei: Local Diamagnetic Current

984
An applied magnetic field causes the electrons present in the molecule to circulate, setting up a local diamagnetic current within the molecule. The local diamagnetic current arising from circulating sigma-bonding electrons induces a magnetic field, Blocal that opposes the applied magnetic field, B0. The effective magnetic field experienced by these nuclei is given by the difference between the applied and local magnetic fields in a phenomenon called local diamagnetic shielding. Essentially,...
984

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescence dye-conjugated magnetic core-shell silica nanoparticles for enhanced nucleic acid visualization.

RSC advances·2025
Same author

Enhanced assembly stability for amine-based cationic glycolipid.

Carbohydrate research·2024
Same author

Highly effective ashless and non-corrosive dimercaptobenzothiadiazole as multifunctional lubricant additives in naphthenic base oil.

RSC advances·2023
Same author

Biomimetic Targeted Theranostic Nanoparticles for Breast Cancer Treatment.

Molecules (Basel, Switzerland)·2022
Same author

Synthesis and biological study of acridine-based imidazolium salts.

RSC advances·2022
Same author

Sugar based cationic magnetic core-shell silica nanoparticles for nucleic acid extraction.

RSC advances·2022

Related Experiment Video

Updated: Sep 24, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

12.2K

Ionic magnetic core-shell nanoparticles for DNA extraction.

Tammar Hussein Ali1,2, Amar Mousa Mandal3, Thorsten Heidelberg1

  • 1Chemistry Department, Faculty of Science, University of Malaya 50603 Kuala Lumpur Malaysia heidelberg@um.edu.my.

RSC Advances
|May 6, 2022
PubMed
Summary

Researchers developed novel magnetic nanoparticles for efficient DNA extraction. These alkylimidazolium-coated nanoparticles offer easy magnetic separation and protect DNA from fragmentation.

More Related Videos

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

6.6K
Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

2.7K

Related Experiment Videos

Last Updated: Sep 24, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

12.2K
A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
05:34

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Published on: April 15, 2021

6.6K
Biofunctionalization of Magnetic Nanomaterials
06:40

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

2.7K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • Magnetic nanoparticles (MNPs) are promising for biomedical applications due to their unique magnetic properties and high surface area.
  • Functionalizing MNPs with specific surface chemistries enables tailored molecular interactions for applications like nucleic acid purification.

Purpose of the Study:

  • To synthesize and characterize magnetic core-shell nanoparticles functionalized with alkylimidazolium for effective DNA extraction.
  • To evaluate the efficiency of DNA binding, magnetic separation, and DNA stability using these novel nanoparticles.

Main Methods:

  • Synthesis of Fe2O3 core-silica shell nanoparticles.
  • Functionalization of silica shell with organic halide and subsequent coating with alkylimidazolium cations.
  • Characterization of nanoparticle size, surface properties, and magnetization.
  • Assessment of DNA extraction efficiency, particle loading capacity, and DNA stability against ultrasound fragmentation.

Main Results:

  • Successfully synthesized ~20 nm magnetic Fe2O3@SiO2 nanoparticles with a magnetization of 25 emu g-1.
  • Achieved effective surface coating with alkylimidazolium cations, facilitating strong interactions with polyanionic DNA.
  • Demonstrated high DNA particle loading capacity (1/30 m%) and efficient DNA isolation using an external magnetic field.
  • Observed that the functionalized nanoparticles stabilized DNA against ultrasound-induced fragmentation.

Conclusions:

  • Alkylimidazolium-coated magnetic core-shell nanoparticles are highly effective for DNA extraction.
  • These nanoparticles offer advantages in terms of high loading capacity, easy magnetic separation, and enhanced DNA stability.
  • The developed material holds significant potential for molecular biology applications, particularly in nucleic acid purification and preservation.