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Updated: Sep 24, 2025

Monitoring Protein Adsorption with Solid-state Nanopores

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

A glass nanopore ionic sensor for surface charge analysis.

Songyue Chen¹, Hong Chen², Jian Zhang³

¹Department of Mechanical and Electrical Engineering, Xiamen University Xiamen 361005 China s.chen@xmu.edu.cn.

|May 6, 2022

Summary

This summary is machine-generated.

This study presents a new method to characterize surface charge in glass nanopores using DNA aptamers for mercury ion detection. This technique can detect mercury ions at very low concentrations (1 pM).

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Area of Science:

Nanotechnology
Analytical Chemistry
Biotechnology

Background:

Surface charge characterization is crucial for nanopore sensing applications.
Existing methods have limitations in sensitivity and specificity for certain analytes.
Mercury ions pose significant environmental and health risks, necessitating sensitive detection methods.

Purpose of the Study:

To develop a novel method for characterizing the inner wall surface charge of glass nanopores.
To functionalize glass nanopores with DNA aptamers for selective mercury ion (Hg²⁺) immobilization.
To achieve highly sensitive detection of mercury ions using nanopore-based surface charge analysis.

Main Methods:

Functionalization of glass nanopores with DNA aptamers forming thymine-Hg²⁺-thymine structures.
Modulation of nanopore surface charge by surface chemistry and varying Hg²⁺ concentrations.
Analysis of surface charge using zeta potential measurements and ionic current rectification ratio in nanopores.

Main Results:

Demonstrated a method for nanoconfined inner wall surface charge characterization.
Successfully immobilized mercury ions using DNA aptamer functionalized nanopores.
Achieved detection of mercury ions down to 1 picomolar (pM) concentration.

Conclusions:

The proposed method enables effective characterization of surface charge within glass nanopores.
DNA aptamer functionalization provides a specific platform for mercury ion capture and detection.
This technique offers a highly sensitive approach for detecting trace amounts of mercury ions, with potential in environmental monitoring and diagnostics.