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3D Nanofiber-Assisted Embedded Extrusion Bioprinting for Oriented Cardiac Tissue Fabrication
Huiquan Wu1, Feng Xu1, Hang Jin1
1Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361102, PR China.
ACS Biomaterials Science & Engineering
|October 19, 2024
Summary
This study introduces 3D nanofiber-assisted embedded bioprinting (3D-NFEP) to create oriented cardiac tissue. The novel method uses aligned nanofibers to guide cell growth, overcoming limitations of traditional bioprinting for tissue engineering.
Area of Science:
- Biotechnology
- Tissue Engineering
- Regenerative Medicine
Background:
- Three-dimensional (3D) bioprinting offers precise control over tissue geometry using cell-loaded bioinks.
- Isotropic culture environments and lack of topographical cues hinder the development of oriented cardiac tissue.
Purpose of the Study:
- To present a novel method, 3D nanofiber-assisted embedded bioprinting (3D-NFEP), for fabricating cardiac tissue with an oriented morphology.
- To overcome the limitations of current bioprinting techniques in achieving oriented cardiac tissue structures.
Main Methods:
- Fabrication of aligned 3D nanofiber scaffolds using divergence electrospinning.
- Utilizing these scaffolds for structural support and topographical cues during the bioprinting of low-viscosity bioinks.
- Assessing cell adhesion and alignment post-hydrogel degradation.
Main Results:
- Aligned 3D nanofiber scaffolds successfully supported the printing of low-viscosity bioink.
- High degrees of cardiomyocyte alignment were observed, adhering to the fibers after hydrogel degradation.
- Demonstrated feasibility for multilayer cell printing.
Conclusions:
- 3D-NFEP is a promising method for bioprinting oriented cardiac tissue using low-viscosity bioinks.
- The technology is expected to advance structured and cardiac tissue engineering applications.
- Aligned nanofibers provide essential structural support and topographical guidance for cardiomyocyte orientation.
