Lignans and clerodane diterpenoids from Tinospora sinensis
Chumao Wen1, Jun Li2, Xiaoshi Xu2
1College of Biomedical Engineering, South-Central University for Nationalities Wuhan 430074 P. R. China.
RSC Advances
|May 6, 2022
Summary
Sixteen lignans and clerodane diterpenoids were isolated from Tinospora sinensis. Compound 20 showed mild nitric oxide production inhibitory activity, suggesting potential therapeutic applications.
Area of Science:
- Phytochemistry
- Natural Products Chemistry
- Pharmacology
Background:
- Tinospora sinensis is a medicinal plant with a history of traditional use.
- Lignans and diterpenoids are classes of natural products known for diverse biological activities.
Purpose of the Study:
- To isolate and characterize chemical constituents from Tinospora sinensis stems.
- To evaluate the nitric oxide production inhibitory activity of the isolated compounds.
Main Methods:
- Extraction of Tinospora sinensis stems using 70% ethanol.
- Structure elucidation of isolated compounds using spectroscopic analyses and chemical methods.
- Assessment of nitric oxide inhibitory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
Main Results:
- Sixteen compounds were isolated, including novel lignans and clerodane diterpenoids.
- Several lignan types, including 7,9'-dinorlignan, dihydrobenzofuran neolignan, and 8-O-4' neolignan, were reported from T. sinensis for the first time.
- Compound 20 demonstrated mild nitric oxide production inhibitory activity (IC50 = 17.43 ± 2.06 μM).
Conclusions:
- The study identified new lignans and diterpenoids from Tinospora sinensis.
- The findings contribute to understanding the chemical diversity of T. sinensis.
- Compound 20's activity suggests potential anti-inflammatory properties warranting further investigation.
