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Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR
Sivaranjan Uppala1, Ayelet Gamliel1, Gal Sapir1
1Department of Radiology, Hadassah Medical Center, Hebrew University of Jerusalem, The Faculty of Medicine Jerusalem Israel rkb@hadassah.org.il.
RSC Advances
|May 6, 2022
Abstract:
A hyperpolarised-NMR acquisition approach that is sensitive to the process of glucose-6-phosphate anomerization is presented. Using selective depolarisation of one of the anomer's signals, it is possible to observe the replenishing of this signal due to the fast anomeric exchange of this compound. The forward to reverse reaction rate constants ratio was ca. 1.6.

