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Light-adapted flicker optoretinograms captured with a spatio-temporal optical coherence-tomography (STOC-T) system
Sławomir Tomczewski1,2,3, Piotr Węgrzyn1,2,4,3, Dawid Borycki1,2
1International Centre for Translational Eye Research, Skierniewicka 10A, 01-230, Warszawa, Poland.
Biomedical Optics Express
|May 6, 2022
Summary
Optoretinography (ORG) measures retinal photoreceptor responses using phase-sensitive optical coherence tomography (OCT). This study demonstrates ORG
Area of Science:
- Ophthalmology
- Biomedical Optics
- Retinal Physiology
Background:
- Electroretinography (ERG) has long been used to assess retinal function.
- Optoretinography (ORG) is a newer technique measuring photoreceptor optical path length changes using phase-sensitive optical coherence tomography (OCT).
- Previous ORG studies primarily used steady light stimuli, with limited investigation into flickering light responses.
Purpose of the Study:
- To investigate the use of spatio-temporal optical coherence tomography (STOC-T) for capturing optoretinograms (ORG) with flickering light stimuli.
- To assess the ability of ORG to detect differences in retinal responses to varying flicker frequencies.
- To evaluate the signal-to-noise ratio (SNR) of ORG in light-adapted versus dark-adapted eyes.
- To demonstrate the spatial mapping of retinal responses to patterned flickering stimuli.
Main Methods:
- Utilized a spatio-temporal optical coherence tomography (STOC-T) system to record optoretinograms (ORG).
- Applied flickering light stimuli to a light-adapted human retina.
- Analyzed photoreceptor optical path length (OPL) modulation amplitudes in response to different flicker frequencies.
- Employed patterned stimuli with spatially varying flicker frequencies.
Main Results:
- Statistically significant differences in photoreceptor optical path length (OPL) modulation amplitudes were detected with varying flicker frequencies.
- Higher signal-to-noise ratios (SNRs) were achieved in light-adapted conditions compared to dark-adapted conditions.
- The study successfully demonstrated the spatial mapping of retinal responses to patterned stimuli with different flicker frequencies.
Conclusions:
- Spatio-temporal OCT-based ORG can effectively measure retinal responses to flickering stimuli.
- ORG provides better SNR in light-adapted retinas and can characterize spatially resolved temporal-frequency responses.
- This technique offers a promising new method for detailed retinal functional analysis.
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