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High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow
Xu-Dong Zhu1, Xiang Shi1, Shu-Wen Wang2
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237 China yhwang@ecust.edu.cn pzuo@ecust.edu.cn.
Abstract:
A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans. In this system, water-in-oil-in-water (W/O/W) droplets, which were ∼12 pL in volume were used as picoliter-reactors for lactic acid fermentation. A fluorescent sensor was developed and used for monitoring pH which indicated the production of lactic acid. After fermentation, fluorescence activated cell sorting was performed with high sensitivity and speed. Using this microfluidic high-throughput screening system, we found a mutant with a yield of 76 g L-1 lactic acid which was 52% higher than its parent strain with a screening throughput exceeding 106 clones per h.
