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Updated: Sep 24, 2025

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
Published on: November 18, 2017
Structural, physicochemical and functional properties of Semen Ziziphi Spinosae protein
Hongyin Zhang1, Shuai Shao2, Rongxin Han1
1Jilin Ginseng Academy, Changchun University of Chinese Medicine Changchun 131200 P. R. China 386759102@qq.com cnzhaodaqing@126.com.
Abstract:
Semen Ziziphi Spinosae (Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou) is a functional food and a traditional Chinese medicine (TCM) in China. Herein, Semen Ziziphi Spinosae protein (SZSP) was prepared by an alkaline extraction and acid precipitation method, of which the structural, physicochemical, functional and emulsion properties were investigated. Results showed that SZSP contained an ideal amino acid composition. The structural properties of the proteins were characterized using Fourier transform infrared spectroscopy (FTIR), relative fluorescence and circular dichroism (CD) spectroscopy analysis. The electrophoresis profiles showed that the main molecular weight of the protein components was about 10-40 kDa and contained some glycoproteins. Differential scanning calorimetry analysis indicated that the denaturation temperature of SZSP was 110.5 °C. The functional properties showed that SZSP has good water and oil absorption capacity, high emulsifying ability and foaming stability. The overall results suggest that SZSP is a promising protein source for the functional food industry.
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