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Updated: Sep 24, 2025
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Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
Published on: November 3, 2013
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EFFECT OF BONE MARROW ASPIRATE IN DENERVATION-INDUCED SKELETAL MUSCLE ATROPHY
Andrii Lysak1, Serhii Savosko2, Sergii Strafun1
1INSTITUTE OF TRAUMATOLOGY AND ORTHOPEDICS OF NATIONAL ACADEMY OF MEDICAL SCIENCES OF UKRAINE, KYIV, UKRAINE.
Wiadomosci Lekarskie (Warsaw, Poland : 1960)
|May 6, 2022
Summary
Bone marrow aspirate cell injections can mitigate muscle atrophy following sciatic nerve damage. Delayed injections at 7 weeks showed a greater benefit in preserving muscle tissue and normalizing biochemical markers.
Area of Science:
- Regenerative Medicine
- Neuroscience
- Skeletal Muscle Biology
Background:
- Sciatic nerve injury leads to significant skeletal muscle atrophy.
- Understanding the temporal effects of cellular therapies on denervated muscle is crucial for treatment development.
Purpose of the Study:
- To investigate the impact of bone marrow aspirate cell (BMAC) injections on muscle changes after sciatic nerve injury.
- To compare the effects of immediate versus delayed BMAC administration on muscle morphology and biochemistry.
Main Methods:
- Sciatic nerve transection and repair (neuroraphy) were performed in 36 rabbits.
- BMAC were injected either directly after neuroraphy or 7 weeks post-surgery.
- Muscle morphology (fiber diameter, collagen) and biochemical markers (catalase, SOD, GPx, TBARS) were analyzed at 8, 12, and 16 weeks.
Main Results:
- Sciatic nerve injury induced muscle fiber atrophy and increased TBARS levels.
- Delayed BMAC injection (7 weeks) attenuated muscle atrophy and delayed the rise in TBARS.
- Direct BMAC injection increased collagen deposition and fibrosis.
Conclusions:
- Sciatic nerve injury causes progressive muscle atrophy and oxidative stress.
- Delayed BMAC injection offers partial protection against muscle atrophy and biochemical alterations.
- BMAC therapy can modulate the degenerative process post-nerve injury, with timing being a critical factor.
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