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Updated: Sep 24, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Direct Sequencing of RNA and RNA Modification Identification Using Nanopore
Thidathip Wongsurawat1, Piroon Jenjaroenpun2, Intawat Nookaew3
1Division of Bioinformatics and Data Management for Research, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. thidathip.won@mahidol.edu.
Abstract:
Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA.
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