Related Experiment Video

Updated: Sep 24, 2025

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
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Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

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Age-related decrease in responsiveness of CD271-positive skin stem cells to growth factors

Hirohiko Akamatsu1, Takaaki Yamada1,2,3, Ayumi Sanada2

  • 1Department of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, Toyoake, Japan.

Experimental Dermatology
|May 7, 2022
PubMed

Insights

Skin stem cell responsiveness to growth factors declines with age, impacting skin repair and healing. Suppressing decreased receptor expression may maintain skin function.

Area of Science:

  • Dermatology
  • Aging Research
  • Stem Cell Biology

Background:

  • Skin stem cell numbers decrease with age, affecting homeostasis and wound healing.
  • Age-related functional decline in stem cells contributes to impaired skin regeneration.
  • Epidermal growth factor/receptor (EGF/EGFR) and fibroblast growth factor-2/receptor (FGF2/FGFR) signaling are crucial for skin repair.

Purpose of the Study:

  • To investigate age-related changes in EGF/EGFR and FGF2/FGFR signaling in skin stem cells.
  • To analyze the impact of aging on the responsiveness of interfolliclicular epidermal stem cells (IFE-SCs) and dermal stem cells (DSCs) to growth factors.

Main Methods:

  • Immunohistological analysis of EGFR and FGFR1 expression in skin samples from different age groups.
  • Isolation and functional assessment of IFE-SCs and DSCs from elderly subjects.
  • Evaluation of stem cell responsiveness to EGF and FGF2 in vitro.

Main Results:

  • Aged skin showed decreased expression of EGFR in IFE-SCs and FGFR1 in DSCs.
  • IFE-SCs and DSCs from elderly subjects displayed reduced responsiveness to EGF and FGF2, respectively.
  • Lowered growth factor signaling correlates with age-related decline in skin regenerative functions.

Conclusions:

  • Reduced responsiveness of skin stem cells to growth factors is a key factor in age-related skin deterioration.
  • Targeting EGFR and FGFR1 expression may offer a strategy to maintain skin homeostasis and improve wound healing in aging individuals.
Keywords:
agningegfrfgfrstem cell

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