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Author Spotlight: Eco-friendly Photoluminescent Textile Authentication with Curcumin
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A Sensitive Spectrofluorimetric Method for Curcumin Analysis.

Anne Boyina Sravani1, Elizabeth Mary Mathew2, Vivek Ghate1

  • 1Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education (MAHE), 576104, Manipal, Karnataka, India.

Journal of Fluorescence
|May 8, 2022
PubMed
Summary
This summary is machine-generated.

A new spectrofluorimetric method offers a simple, fast, and validated way to quantify curcumin (CUR) in various products. This method shows comparable accuracy to HPLC and can be used for quality control in healthcare and food industries.

Keywords:
96 well platesCost-effectiveCurcuminHPLCSpectrofluorimetric methodValidation

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Curcumin (CUR), a natural compound from Curcuma longa, has diverse applications including pharmaceuticals, food, and dyes.
  • CUR is under investigation for cancer treatment and exhibits anti-inflammatory, anti-microbial, and anti-oxidant properties.
  • Accurate quality control of CUR is essential due to its wide-ranging applications and therapeutic potential.

Purpose of the Study:

  • To develop and validate a rapid, simple, and time-saving 96-well plate spectrofluorimetric method for CUR determination.
  • To compare the performance of the developed spectrofluorimetric method with the established High-Performance Liquid Chromatography (HPLC) technique.
  • To assess the applicability of the new method for CUR quantification in complex matrices like nanoparticles.

Main Methods:

  • Development of a 96-well plate spectrofluorimetric assay for CUR.
  • Validation of the assay for linearity, accuracy, and precision.
  • Comparative analysis with HPLC using Bland-Altman analysis.
  • Application of the method for CUR determination in solid lipid nanoparticles (SLNs) and chitosan nanoparticles (Chi-NPs).

Main Results:

  • The spectrofluorimetric method demonstrated linearity from 15 to 3900 ng/mL (R² ≥ 0.9983).
  • Detection and quantification limits were 7 and 15 ng/mL, respectively, showing high sensitivity.
  • Bland-Altman analysis confirmed good agreement between the spectrofluorimetric and HPLC methods.
  • The method was successfully applied to quantify CUR in SLNs and Chi-NPs.

Conclusions:

  • A validated, rapid, and simple 96-well plate spectrofluorimetric method for CUR quantification has been developed.
  • This method offers a viable alternative to HPLC for CUR analysis in pharmaceutical and food products.
  • The developed assay is suitable for routine quality control and analysis of CUR in nanoparticle formulations.