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Related Concept Videos

Colloids03:22

Colloids

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Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
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Colloidal precipitates

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The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
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Coagulation01:06

Coagulation

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Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
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Related Experiment Video

Updated: Sep 24, 2025

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
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Multi-species colloidosomes by surface-modified lactic acid bacteria with enhanced aggregation properties.

Xiaoyi Jiang1, Helle Jakobe Martens2, Elhamalsadat Shekarforoush1

  • 1University of Copenhagen, Department of Food Science, Rolighedsvej 30, DK-1958 Copenhagen, Denmark.

Journal of Colloid and Interface Science
|May 8, 2022
PubMed
Summary

Surface modification of lactic acid bacteria creates stable microbubbles that transform into water-filled colloidosomes. These bacterial colloidosomes can be further engineered for structured materials and controlled substance release.

Keywords:
AdsorptionAggregationColloidosomeLactic acid bacteriaLayer-by-layerMicrobubblesOctenyl succinic anhydride

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Area of Science:

  • Biomaterials science
  • Microbiology
  • Surface chemistry

Background:

  • Lactic acid bacteria (LAB) possess unique surface properties.
  • Microbubble stabilization and transformation into functional structures remain challenging.
  • Layer-by-Layer (LBL) assembly offers a route for material functionalization.

Purpose of the Study:

  • To enhance LAB adsorption and aggregation at the air-water interface.
  • To stabilize microbubbles formed by modified LAB.
  • To create and characterize water-filled colloidosomes from modified LAB, with potential for LBL modification.

Main Methods:

  • Surface modification of LAB using octenyl succinic anhydride (OSA).
  • Characterization of bacterial physicochemical properties (WCA, aggregation, zeta potential).
  • Microbubble stabilization and colloidosome formation.
  • LBL deposition for colloidosome reinforcement (polymer/hybrid).
  • Microscopy (SEM, CLSM) and permeability studies.

Main Results:

  • OSA-modified LAB showed enhanced adsorption and aggregation at the air-water interface, stabilizing microbubbles.
  • Stable, water-filled colloidosomes were formed without shell disintegration.
  • Colloidosomes could be further reinforced using LBL with polymer or hybrid formulations.
  • Hybrid LBL coating using two bacterial species resulted in low shell porosity.

Conclusions:

  • Surface modification of LAB is a viable strategy for creating stable bacterial colloidosomes.
  • Bacterial colloidosomes can be engineered for controlled porosity and potential applications.
  • This approach enables the organization of living bacteria into structured materials and controlled encapsulation/release systems.