Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pathogen to read: a rapid, automated workflow for public health-ready microbial sequencing.

BMC genomics·2026

Same author

Comparative Evaluation of HepG2 Spheroid Generation Methods for Toxicology.

Cellular and molecular bioengineering·2026

Same author

Integrated Experimental-Computational Framework for Drug Transport Quantification in 3D <i>Microtissues</i>™.

Micromachines·2026

Same author

HIV diagnostic implementation in the Dominican Republic: an in-depth qualitative context analysis for health technology developers.

BMJ open·2026

Same author

Cell2Read: an automated workflow to generate sequencing-ready DNA libraries from human cell suspensions.

Biology methods & protocols·2025

Same author

Developing Topics.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Search research articles

Related Experiment Video

Updated: Sep 24, 2025

Real-time Tracking of DNA Fragment Separation by Smartphone

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

Sequence to size-based separation using microfluidic electrophoresis for targeted cell-free DNA analysis.

Lindsay Schneider¹, Anubhav Tripathi¹

¹Center for Biomedical Engineering, School of Engineering, Brown University, 184 Hope Street, Providence, RI, 02912, USA.

Analytical Biochemistry

|May 8, 2022

Summary

This study introduces a novel method for DNA analysis, enabling the separation of same-sized DNA fragments based on specific sequences. This technique allows for multiplexed analysis of short DNA sequences without fluorescence detection.

Keywords:

DNA analysis Electrophoresis Separation

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Related Experiment Videos

Last Updated: Sep 24, 2025

Real-time Tracking of DNA Fragment Separation by Smartphone

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Accurate identification and separation of DNA fragments are crucial for molecular diagnostics.
Existing methods often rely on fluorescence detection, limiting multiplexing capabilities and requiring optimization.
Targeted analysis of short DNA sequences, such as cell-free DNA, presents unique challenges.

Purpose of the Study:

To develop and demonstrate a novel, sequence-specific method for differentiating and separating DNA fragments of identical size.
To enable multiplexed analysis of targeted DNA sequences without the need for fluorescence detection.
To establish a robust and simple method for the targeted analysis of short DNA sequences.

Main Methods:

Sequence-specific DNA manipulation involving denaturation, hybridization, and ligation.
Microfluidic electrophoresis for fragment separation.
Universal amplification and purification steps.

Main Results:

Successful separation of multiple 150 bp target DNA sequences into distinct peaks (230 bp to 330 bp) using microfluidic electrophoresis.
Demonstrated a limit of detection of 3 pg (approximately 1.825 x 10^7 copies) of input DNA with 20 PCR cycles.
Validated the method for analyzing short DNA sequences, including cell-free DNA, extracted from plasma samples.

Conclusions:

The developed method offers a simple, robust approach for targeted analysis of short DNA sequences.
This technique facilitates multiplexed analysis and mutation detection without requiring multiple fluorophores or extensive optimization.
The method holds significant potential for various molecular diagnostic applications, including the qualitative evaluation of specific target sequences.