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Updated: Sep 24, 2025
Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells
Published on: May 12, 2023
In situ fluorescence imaging reveals that mitochondrial H2O2 mediates lysosomal dysfunction in depression
Xin Wang1, Qi Ding1, Ying Tian1
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, P. R. China. lip@sdnu.edu.cn.
Abstract:
We constructed two fluorescent probes for monitoring H2O2 in the mitochondria and lysosomes. Fluorescence imaging reveals that mitochondrial H2O2 mediates reduced GCase activity in lysosomes in the brains of mice with depression. This work provides a powerful tool for understanding the pathogenesis of depression.
