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Acid-activatable inactive renin in cat plasma and kidney
Summary
Cat inactive renin, found in plasma and kidneys, behaves like human prorenin. Acid dialysis demonstrated reversible and irreversible activation, suggesting it is indeed prorenin.
Area of Science:
- Biochemistry
- Renal Physiology
Background:
- Inactive renin has been detected in feline plasma and kidney extracts.
- Its characteristics are similar to human plasma prorenin, with minor differences in pH optimum for activation.
Purpose of the Study:
- To investigate whether feline inactive renin is prorenin.
- To examine the acid-activation properties of feline inactive renin.
Main Methods:
- Dialysis of partially purified cat renal inactive renin to pH 3.3.
- Acidification of plasma followed by dialysis to pH 7.4 at 4°C.
- Assessment of angiotensinogen stability under acid dialysis conditions.
Main Results:
- Cat inactive renin exhibited reversible acid activation upon dialysis to pH 3.3.
- Acidification followed by dialysis to pH 7.4 resulted in irreversible activation, mirroring human prorenin behavior.
- Cat angiotensinogen remained unaffected by acid dialysis, unlike its human counterpart.
Conclusions:
- Feline inactive renin is highly likely to be prorenin.
- The acid-activation properties strongly support the identification of cat inactive renin as prorenin.
- This finding contributes to comparative studies of the renin-angiotensin system in different species.