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Related Experiment Video

Updated: Sep 24, 2025

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Engineering Aptamers with Selectively Enhanced Biostability in the Tumor Microenvironment.

Sitao Xie1,2, Zhimin Wang2, Ting Fu1,2

  • 1The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.

Angewandte Chemie (International Ed. in English)
|May 10, 2022
PubMed
Summary

Researchers developed a new aptamer stabilization strategy that protects against degradation in healthy organs but enhances stability within tumors. This improves cancer theranostics by prolonging retention in tumors while minimizing side effects.

Keywords:
AptamersBiostabilityMolecular EngineeringNucleic AcidsTumor Microenvironment

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanomedicine

Background:

  • Aptamers show promise for cancer theranostics but face challenges with in vivo stability.
  • Current stabilization methods may cause prolonged retention in healthy organs, leading to side effects.

Purpose of the Study:

  • To develop an environment-responsive strategy for selective aptamer stabilization in the tumor microenvironment (TME).
  • To enhance aptamer biostability and retention specifically within tumors, reducing off-target accumulation.

Main Methods:

  • Designed an ATP-responsive protection (ARP) module for aptamer end-extension.
  • Utilized ATP incorporation to protect aptamers from nuclease degradation.
  • Evaluated in vivo tumor accumulation and retention of the ARP-aptamer probe.

Main Results:

  • The ARP-aptamer probe accumulated effectively in tumors through molecular recognition.
  • Demonstrated selectively prolonged retention time in tumors.
  • Showed rapid digestion of the aptamer in healthy organs, minimizing off-target effects.

Conclusions:

  • The developed ARP strategy offers selective enhancement of aptamer biostability in the TME.
  • This approach provides a new paradigm for organ-specific nucleic acid-based imaging and therapeutic agents.
  • Minimizes side effects by reducing aptamer retention in healthy tissues.