Consistency Analysis of 3 Detection Systems for Measuring Serum C-Reactive Protein

Area of Science:

• Clinical chemistry
• Laboratory diagnostics
• Biomedical instrumentation

Background:

• C-reactive protein (CRP) is a crucial biomarker in clinical diagnostics.
• Variability in CRP measurements across different analytical platforms necessitates consistency evaluation.
• Turbidimetry is a common method for serum CRP detection.

Purpose of the Study:

• To assess the inter-instrument consistency of serum C-reactive protein (CRP) measurements.
• To compare CRP detection using Mindray BC-5390, Mindray BC-6800, and Johnson Vitros5600 systems.
• To evaluate the interchangeability of Mindray BC-5390 and BC-6800 for routine blood tests.

Main Methods:

• Comparative analysis of CRP measurements from 847 patients.
• Utilized turbidimetric assays on Mindray BC-5390, Mindray BC-6800, and Johnson Vitros5600 analyzers.
• Employed Pearson correlation, Passing-Bablok, and weighted Deming analyses for consistency assessment.

Main Results:

• Significant differences in CRP values were observed among the three tested instruments (P<0.001).
• Mindray BC-5390 demonstrated high consistency with both BC-6800 (R=0.994) and Vitros5600 (R=0.983).
• A constant deviation was noted between BC-6800 and Vitros5600 CRP measurements (R=0.994); BC-5390 and BC-6800 were interchangeable for routine blood tests.

Conclusions:

• The Mindray BC-5390, BC-6800, and Johnson Vitros5600 exhibit varying levels of consistency for CRP detection.
• The BC-5390 and BC-6800 analyzers are interchangeable for routine blood tests.
• Findings provide valuable reference data for selecting appropriate CRP detection instruments in clinical settings.