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Updated: Sep 23, 2025
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Isolation, identification and bioactivities of abietane diterpenoids from Premna szemaoensis
De-Bing Pu1,2,3, Ting Wang4, Xing-Jie Zhang1
1Key Laboratory of Medicinal Chemistry for Natural Resource of Ministry of Education, School of Chemical Science and Technology, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University Kunming 650091 People's Republic of China lixiaoli@ynu.edu.cn xiaoweilie@ynu.edu.cn +86 871-665033214 +86 871-67357014 +86 871-665033214 +86 871-67357014.
Abstract:
Investigation of the leaves and stems of Premna szemaoensis resulted in the isolation of twelve new abietane diterpenoids, szemaoenoids A-L (1-12), together with four known abietane diterpenoids (13-16). The structures involved two rearranged-abietane skeletons: 17(15 → 16)-abeo-abietane (7, 10-12, 14 and 15) and 17(15 → 16),18(4 → 3)-diabeo-abietane (1-6, 13 and 16). The structures of the new compounds were established mainly by analyzing NMR and HRESIMS data. The absolute configurations of 1, 3 and 10 were confirmed by single crystal X-ray diffraction analysis. In bioactivity assays, compounds 11, 12, 14 and 15 were active against two human colon cancer cell lines (HCT-116 and HT-29) with IC50 values ranging from 8.8 to 34.3 μM, and compounds 10, 13 and 14 exhibited effective free radical scavenging activity with IC50 values ranging from 35.6 to 41.5 μM by DPPH experiment.
