Preparation of a balsa-lysozyme eco-friendly dressing and its effect on wound healing

This study aims to prepare an eco-friendly dressing using a balsa derived membrane with lysozyme included for anti-bacterial purposes. The balsa-lysozyme was prepared using delignification (control) and dopamine (group A) methods for mussel-inspired adhesion of 5, 10, 15 and 20 mg ml^-1 lysozyme (groups B, C, D and E). Fourier infrared spectra and the contact angle test showed that lysozyme adhered to the membrane. With increasing concentration of lysozyme, the drug-loading rate of balsa-lysozyme increased and the encapsulation efficiency decreased (P < 0.05). The cumulative release percentages after 72 h were 80.7%, 90.6%, 91.4%and 92.3% in groups B, C, D and E, respectively. There was a significant in vitro antibacterial effect against both E. coli and S. aureus. The cytotoxicity of the wood dressing was not detected until day 7. On day 7, the healing rates were 30.7%, 38.3%, 50.7%, 61.2%, 61.9% and 62.4% for the control, A, B, C, D and E group (P < 0.05). Similarly, the lengths of the new epithelium were 631.7 μm, 702.5 μm, 759.4 μm, 825.3 μm, 831.7 μm and 836.6 μm for the control group, A, B, C, and D, E respectively (P < 0.05). Furthermore, PCNA and CD31 expression indicated enhanced cell proliferation and angiogenesis in the C, D and E group (P < 0.05).