Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multi-Stimuli Responsive Viologen-Crosslinked Eutectogels Derived From Natural Deep Eutectic Solvents for Information Security and Motion Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Macromolecules with Tunable Fluorescence via Photochemical Step-Growth Polymerization.

ACS macro letters·2026

Same author

Visible Light Photo-Cross-Linked Thermogel─A Single-Component Hybrid Supramolecular-Covalent Hydrogel for Sustained Drug Release.

ACS applied materials & interfaces·2026

Same author

Understanding Wavelength-Dependent Photopolymerizations via Nano-Second Resolved Transient Spectroscopy.

Journal of the American Chemical Society·2026

Same author

Following the formation of single-chain nanoparticles generated by interblock crosslinking within diblock copolymers: a Monte Carlo simulation study with adjustable interaction strength between the blocks.

Soft matter·2026

Same author

Wavelength-Dependent 3D Printing: Introducing 3D Printed Action Plots.

Advanced materials (Deerfield Beach, Fla.)·2026

Same journal

Bi-modified Ni<sub>3</sub>S<sub>2</sub> promotes selective nitrite-to-hydroxylamine reduction for cyclohexanone oxime synthesis.

Chemical communications (Cambridge, England)·2026

Same journal

Cyclable manganese inventory as a mechanistic descriptor for electrolyte design in rechargeable aqueous Zn-MnO<sub>2</sub> batteries.

Chemical communications (Cambridge, England)·2026

Same journal

Cobalt-iron layered double hydroxides with ligand modification for boosting glycerol electrooxidation-assisted hydrogen production.

Chemical communications (Cambridge, England)·2026

Same journal

Beyond the vacuum: modeling the solid-liquid interface for gas-involving electrocatalysis.

Chemical communications (Cambridge, England)·2026

Same journal

Alkaline-earth carbonate-supported Ru for quinoline hydrogenation: enhanced H<sub>2</sub> activation <i>via</i> electronic metal-support interaction.

Chemical communications (Cambridge, England)·2026

Same journal

Mitigating ionic conductivity limitations <i>in operando</i> electrochemical X-ray photoelectron spectroscopy.

Chemical communications (Cambridge, England)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 23, 2025

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Green light enabled Staudinger-Bertozzi ligation.

Kubra Kalayci^1,2, Hendrik Frisch^1,2, Christopher Barner-Kowollik^1,2,3

¹Centre for Materials Science, Queensland University of Technology, 2 George Street, Brisbane, QLD 4000, Australia. christopher.barnerkowollik@qut.edu.au.

Chemical Communications (Cambridge, England)

|May 11, 2022

Summary

This summary is machine-generated.

We developed a green light-activated Staudinger-Bertozzi ligation by uncaging a phosphine using a coumarin derivative. This method enables visible light-induced modification of polymers and patterning of surfaces.

More Related Videos

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

Published on: December 30, 2016

Related Experiment Videos

Last Updated: Sep 23, 2025

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

Published on: December 30, 2016

Area of Science:

Organic Chemistry
Polymer Science
Materials Science

Background:

The Staudinger-Bertozzi ligation is a versatile click chemistry reaction.
Visible light-triggered reactions offer spatiotemporal control in chemical synthesis.

Purpose of the Study:

To develop a visible light-induced Staudinger-Bertozzi ligation.
To enable photo-uncaging of a triphenylphosphine moiety using a coumarin derivative.
To demonstrate applications in materials science.

Main Methods:

Photo-uncaging of a triphenylphosphine using a photolabile coumarin derivative.
Action plot study to determine optimal wavelengths for uncaging and ligation.
Endgroup modification of water-soluble poly(ethylene glycol).
Green light-induced patterning of solid substrates.

Main Results:

The Staudinger-Bertozzi ligation was successfully induced by visible green light (λ < 550 nm).
The photo-uncaging and subsequent Staudinger reaction were confirmed.
The method was applied to modify poly(ethylene glycol) endgroups.
Patterning of solid substrates using green light was achieved.

Conclusions:

A novel visible light-induced Staudinger-Bertozzi ligation has been established.
The reaction is efficiently triggered by green light, offering precise control.
The methodology is applicable for polymer modification and surface patterning in materials science.