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Apoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned

Arnav Lal1,2, Allison Kranyak1, Jonathan Blalock1

  • 1Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, 701 Grove Road, Greenville, SC, 29605, USA.

Journal of Assisted Reproduction and Genetics
|May 11, 2022
PubMed
Summary

Analyzing apoptotic gene expression in blastocoel fluid from IVF embryos shows promise for predicting implantation success. This novel method offers insights beyond traditional embryo biopsy, aiding in successful IVF outcomes.

Keywords:
AneuploidyApoptosisBlastocoel fluidDiagnosticEmbryo selectionIVFSelf-correctionqPCR

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Area of Science:

  • Reproductive biology
  • Genetics
  • Biomarker discovery

Background:

  • Conventional embryo biopsy for genomic analysis has limitations in predicting implantation.
  • Transcriptomic biomarkers offer potential for enhanced implantation prediction in human IVF embryos.
  • Assessing gene expression in blastocoel fluid presents a novel, non-invasive approach.

Purpose of the Study:

  • To demonstrate proof-of-concept for analyzing embryo gene expression using blastocoel fluid-conditioned media via qPCR.
  • To examine the comparative presence of apoptotic genes as potential biomarkers.
  • To correlate gene expression patterns with embryo ploidy status and clinical outcomes.

Main Methods:

  • Blastocoel fluid-conditioned media were collected from 19 human IVF embryos (11 euploid) after trophectoderm biopsy.
  • Quantitative PCR (qPCR) was used to assess apoptotic gene expression.
  • Statistical analyses included Wilcoxon Signed-Ranks test, ANOVA, and chi-square tests.

Main Results:

  • Significantly higher overall apoptotic gene expression was observed in euploid versus aneuploid embryos (p=0.001).
  • Higher expression of pro-apoptotic genes correlated with successful implantation (p=0.045).
  • Specific genes, BBC3 (p=0.012) and BCL2L13 (p=0.003), showed significantly higher expression in euploid embryos.

Conclusions:

  • Qualitative analysis of gene expression in blastocoel fluid-conditioned media is feasible.
  • Global and gene-level expression trends in blastocoel fluid may correlate with clinical outcomes.
  • Blastocoel fluid analysis shows potential as an additional predictive tool for IVF cases, warranting larger investigations.